The SKC BioStage
®
single-stage viable cascade impactors operate on the principle
of inertial impaction and meet NIOSH Method 0800 and 0801 requirements and
ACGIH recommendations for sampling viable microorganisms including bacteria
and fungi. Made of precision-tooled autoclavable aluminum, sampler components
are precision-threaded together for a positive seal to ensure sample integrity. Air is
drawn through the impactor where particles are impacted onto an agar collection
medium for culture, count, and identifi cation. BioStage provides performance
equivalent to the Andersen N-6 and Aerotech A6 (see reference on page 7). Two
models of the BioStage Impactor are available.
Standard BioStage contains a 400-hole jet classifi cation stage and
is operated at 28.3 L/min
BioStage 200 contains a 200-hole jet classifi cation stage and is
operated at 14.15 L/min
Specifi cations
Flow Rate: Standard BioStage: 28.3 L/min
BioStage 200: 14.15 L/min
Material: Inlet cone and
base plate: Precision-tooled autoclavable
aluminum
O-rings: Duro 50, BUNA-N (not autoclavable)
Jet Classifi cation Stage: Standard BioStage: 400 holes (0.25-mm hole diameter)
BioStage 200: 200 holes (0.25-mm hole diameter)
Median Cut-point (D
50
): 0.6 μm
Sample Media: 15 x 90 to 100-mm agar plates*
Analysis: Colony culture
†
Tubing: 1/4-inch ID
* Consult a laboratory for information on appropriate agar choice.
† In situations where spore counts are high, positive-hole correction should be used. See Macher,
J., "Positive-hole Correction of Multiple-jet Impactors for Collecting Viable Microorganisms,"
American Industrial Hygiene Journal, 50 (11), 1989, pp. 561-568, available at www.skcinc.com/
pdf/Multiple_Jet_Impactors.pdf
Sample Media
Use appropriate agar in a 15 x 90 to 100-mm agar plate with the Standard BioStage
or the BioStage 200. Plastic or glass agar plates can be used. Verify with your agar
supplier that the plates contain the proper volume of agar to achieve the appropriate
agar height to maintain impactor cut-points. Store agar plates as directed by the
supplier.
Suggested Media
For Bacteria: Tryptic Soy Agar (TSA) or Blood Agar Plates (BAP)
For Fungi: Potato Dextrose Agar (PDA), Malt Extract Agar (MEA),
Dichloran Glycerol 18 Agar (DG-18), or Corn Meal Agar (CMA)
For information on laboratories that can provide agar plates and analyze
samples, see Laboratories at www.skcinc.com.
Operating Instructions
863 Valley View Road, Eighty Four, PA 15330 USA
BioStage Impactors
Kommentare zu diesen Handbüchern